Kinetic Induction

"PreHistoric Megalithic Complex in Karelia, Russia - Vottovaara Mountain"
"...The Vottovaara Mountain, situated in the Republic of Karelia of Russia, is a place revered for its powerful energy and worshipped as a sacred site for thousands of years by ancient Saami tribes and shamans. The site has numerous strange megalithic structures and ruins, which many believe could not have occurred naturally. For example, there are approximately 1600 sacred stones called "seids" which are arranged here in a mysterious order. There’s a structure called the well, which locals believe is an artificially constructed ancient megalithic water reservoir..."

"THE MOST BIZZARE PIECE OF HISTORY YOU WOULD EVER SEE"
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"Old World Water Supply"
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"OLD WORLD FACTORY PROCESSING"

star-protocols.cell.com/protocols/1179
"Optimized electroporation of CRISPR-Cas9/gRNA ribonucleoprotein complex for selection-free homologous recombination in human pluripotent stem cells"
"Summary
Selection-free, scarless genome editing in human pluripotent stem cells (PSCs) by utilizing ribonucleoprotein (RNP) of CRISPR-Cas9 is a useful tool for a variety of applications. However, the process can be hampered by time-consuming subcloning steps and inefficient delivery of the RNP complex and ssDNA template. Here, we describe the optimized protocol to introduce a single nucleotide change or a loxP site insertion in feeder-free, xeno-free iPSCs by utilizing MaxCyte and 4D-Nucleofector electroporators.

For complete details on the use and execution of this protocol, please refer to Kagita et al. (2021) and Xu et al. (2019)..."
"Before you begin
The discovery of CRISPR-Cas9 has made tremendous progress in various research areas especially for genetic functions, disease modeling, and gene therapy (Dastidar et al., 2018; Ran et al., 2013; Soldner and Jaenisch, 2018; Zhong et al., 2020). This technology is widely applied to human PSCs, which have a great potential for regenerative medicine and cell therapies. With this in mind, the protocol utilizes feeder-free, xeno-free human PSCs cultured on recombinant Laminin-511 (iMatrix-511) with StemFit AK02N (or AK03N) media.

For successful genome editing, the method to introduce Cas9/gRNA complex into cells is critical. Ribonucleoprotein (RNP) form of delivery has several advantages over the DNA/RNA form (Gee et al., 2020; Kim et al., 2014; Paquet et al., 2016; Richardson et al., 2016; Zuris et al., 2015), since the RNP functions immediately, degrades quickly, and is relatively smaller than plasmid DNA or mRNA. Furthermore, the pre-formed Cas9/gRNA RNP complex can evade the inhibition by cellular mRNA (Kagita et al., 2021).

In addition to Cas9/gRNA, single-stranded oligodeoxynucleotides (ssODNs) must be available at the DNA repair site as a template for homology directed repair (HDR) pathway. In this protocol, we introduce Cas9/gRNA RNP complex and ssODN together into human PSCs by utilizing MaxCyte or 4D-Nucleofector electroporators, which were superior for inducing HDR.

Also, this protocol can be applied for single nucleotide editing for disease modeling and correcting mutations in PSCs, since more than half of disease-associated genetic changes are single nucleotide polymorphisms (Bamshad et al., 2011; Collins et al., 1997). Moreover, a 34 bp loxP sequence can be inserted for further genomic engineering by Cre recombination (Branda and Dymecki, 2004; Sauer and Henderson, 1988). Additionally, by simply omitting an ssODN, a small (∼10 nt) indel may be introduced for knocking out a target gene.

Preparation of human iPSCs (feeder-free, Xeno-free condition)..."

#Gnosisosis: "The doctrine of Lucifer, or Luciferianism, is a belief system inspired by Gnosticism, based various religions and myths associated with Venus, the morning star, traditionally known as the "light bearer" or Lucifer", "not detested but revered", inversions of reality, we have all been lied-to, beginning 1666, getting a big boost December 16, 1811... -1812Project:

...nepotism of inheritors have infiltrated every institution, hiding behind our religions, playing both sides of every "wars, plagues. famine, repeat" cycle of passed down ignoramce, just to further desttroy our forefather's advancements, leaving this entire population suffering devolution, by design...

...& IF we're unable to realize how we've all been 'taken for a ride', IF we allow this behavior to continue, in all truth, this 'human-race' has beeen run, exactly what all this deviant behavior has been attempting to achieve,& along with electrical-disease, parasites, environmental-toxins, poisons,.. all the psyops, prropaganda has been humanities greatest downfall...

-rid the falsified-narratives, relive reality- -remember-
Kinetic Induction: lnkd.in/gvNyUumM
"bring back our lost Antiquitech, find the truths behind the myths"
www.linkedin.com/posts/tony-hood-56419040_the-luciferian-doctrine-explained-robert-activity-7237860023979167744-f9fI?utm_source=share&utm_medium=member_desktop

"The Luciferian Doctrine Explained - ROBERT SEPEHR"

Anthropology News: "old world faces in some interesting places". "behind enemy lines", still battling "The War of 1812Project", even though Tesla tried, Morgan's buddies told him "can't meter, don't want", steady destruction of olde-world tech, torching cathedrals to bring down spiral-cathodes, cutting electrical-cables off tops of pyramids... ..."bomb Dresden!"...

...starforts aren't "military", they charge/d electrolytic-waters...
...our cities had solid gold statues, that were purposely melted, FDR made gold illegal, told copper was outdated, installing PVC, mercury's toxic, removing from mooring-stations, that were extracting hydrogen, helium for airships, lighthouses are not just for boats, & we were all informed "helium is just so rare, okay for party-balloons", although second most abundant gas...

...all of humanity has been lied to, beginning December 16, 1811, & IF we can't get our pilfered technologies back, this human-race has been run,.. so, what do you believe our chances, of seeing our pyramidal-complexes once again lit-up... -how devolved-
-all of what was recently lost- -remember-
Kinetic Induction: lnkd.in/gvNyUumM
"bring back our lost Antiquitech, find the truths behind the myths"
www.linkedin.com/posts/tony-hood-56419040_old-world-faces-activity-7238990020815060993-MpZY?utm_source=share&utm_medium=member_desktop

"Old World Faces"
"...Looking into Native American Faces in Old World Places?"?

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"It’s Reality! Scientists Finally Discover The TRUTH About Ancient Technology!
"...Discover the shocking truth about ancient technology and how advanced civilizations may have harnessed wireless electricity. This video uncovers the hidden connections between ancient structures, from pyramids to cathedrals, and their mysterious architectural designs. Explore the possibility of a global civilization reset and what it means for our understanding of history..."
Kinetic Induction: lnkd.in/eKp7YqCU
www.linkedin.com/posts/tony-hood-56419040_its-reality-scientists-finally-discover-activity-7241494473313071105-Yabu?utm_source=share&utm_medium=member_desktop

www.sciencealert.com/first-of-its-kind-dna-computer-can-both-store-data-and-solve-problems
"First of Its Kind DNA Computer Can Both Store Data And Solve Problems
Tech
17 September 2024
ByDavid Nield":

"...For billions of years, life has used long molecules of deoxyribonucleic acid, or DNA, to store information and solve problems.

Today engineers are putting their own spin on DNA computing, to both record data and serve as biological computers, yet until now they've struggled to design a synthetic system that can store and perform tasks at the same time.


New research has now demonstrated it's possible to package and present DNA so it can manage both, providing a full suite of computing functions out of strings of nucleic acids. Specifically, we're talking about storing, reading, erasing, moving, and rewriting data, and handling these functions in programmable and repeatable ways, similar to how a conventional computer would operate.

Researchers from North Carolina State University (NC State) and Johns Hopkins University demonstrated in experiments that their novel nucleic acid scaffold serves as proof of the versatility of DNA computing, and has the potential to build incredibly compact biological machines...

..."It's been thought that while DNA data storage may be useful for long-term data storage, it would be difficult or impossible to develop a DNA technology that encompassed the full range of operations found in traditional electronic devices," says NC State molecular biologist Albert Keung.

"We've demonstrated that these DNA-based technologies are viable, because we've made one."

DNA acts as a codebase for living organisms, providing molecular templates for biological structures and processes. Yet in theory, the chemical strings could represent just about any sequence of information. Scientists have been developing methods for cramming all kinds of data into free-floating strands of DNA for years.

By instead storing the molecules on tiny tree-like structures called dendricolloids, the researchers were able to not only store, but also continuously edit their code far more easily...

...Key to the system is the way that the DNA information can be distinguished from the dendricolloid nanofibers it's stored on: this enables the data to be copied into RNA (ribonucleic acid) form to be processed, or specific areas of DNA to be rewritten, without damaging the original DNA 'files' or the storage medium.

Even better, the branching dendricolloid scaffold helps preserve the DNA information as well, compared with polymers in solution. Accelerated aging analysis suggested strands stored on dendricolloids at a temperature of around 4 degrees Celsius (about 39 degrees Fahrenheit) would have a half-life of millennia. At lower temperatures, it could be millions of years.

Using a system like this, data that would fill up a thousand laptops can be reliably stored in a space the size of a pencil eraser, Keung says, allowing for the long-term storage of vast databases.

"It essentially allows us to conduct the full range of DNA data storage and computing functions," says NC State chemical engineer Kevin Lin.

While we're still a long way from these DNA computers finding widespread use, this shows that it can be done. Already, the biological machine has been able to solve simple chess and sudoku problems – hardly in the realms of supercomputers but certainly impressive for microscopic molecules.

It may not be particularly powerful or particularly fast right now, but the promise of DNA storage and computing is cramming vast amounts of information into a super-small space, on a medium that could potentially outlast whole civilizations...

"We wanted to develop something that would inspire the field of molecular computing," says Keung. "And we hope what we've done here is a step in that direction."

The research has been published in Nature Nanotechnology..."